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it is also a major NAD + consumer with a lower K m than most NAD + -consuming enzymes 40 and is implicated in the decline of NAD + levels observed during aging and disease 50–52

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First, the participant answers two questionnaires (short-form McGill Pain Questionnaire (MPQ) 27 and RAND-36 (RAND-36 Health-Related Quality of Life)), 28 then stimulation tests (cold pressor test and pressure algometry) 29–33 are performed followed by pain rating (NRS) 34 immediately after 1 min and after 3 min